Palo Alto Veterans Institute for Research Life Sciences Research Assistant I (MAL1701.17) in Palo Alto, California

The Palo Alto Veterans Institute for Research (PAVIR) is seeking a Life Sciences Research Assistant position in the Maltzman laboratory, part of the VA Palo Alto Healthcare System and Stanford University, to support the cellular and molecular investigation of T lymphocyte biology under the supervision of Principal Investigator - Jonathan Maltzman, M.D., Ph.D. The Research Assistant will study the immune system using mouse models and human samples and will use flow cytometry and next generation sequencing approaches. The successful candidate will be a highly-motivated individual with experience in molecular biology. This is a regular, full time (40 hours/week), non-exempt (hourly-paid) position. PAVIR is a nonprofit foundation affiliated with the Veterans Affairs Palo Alto Health Care System (VAPAHCS). As a condition of employment, all PAVIR employees are required to have an approved appointment with VAPAHCS and complete a background check before they can commence work. Duties:Maintain mouse colony, including PCR based genotyping and database entryPerform isolation of lymphocytes from genetically modified miceConduct In vitro approaches to characterize T lymphocyte functionOrder supplies, maintain compliance records, aid in research protocol developmentPerform flow cytometric analysis of mouse and human lymphocytesAssist other laboratory personnel as neededMaintain a laboratory notebook and spreadsheets and prepare progress reports and summaries; perform other duties, as assignedRequirements: Education:Required: Bachelor degree (or equivalent combination of education and experience) in biological or biomedical sciences or a related field. Experience:Required: At least six (6) months of related experience (may include undergraduate lab courses and academic lab undergraduate research experience) Desired: One year of related lab/bench experience; experience with nucleic acid extraction, amplification, quantitation, and other manipulations, including agarose gel electrophoresis is strongly desired; experience working with mice will be considered a strong plus; experience with RNA extraction a plus. Knowledge / Skills / Abilities:Knowledge of immunology and/or molecular biologyPrevious experience in a variety of research techniques/methods and follow-up data collection is strongly preferredKnowledge of research procedures and protocols gained through education and/or experienceKnowledge of lab safety proceduresKnowledge of Microsoft Office (Word, Excel, Outlook)Previous experience with mouse genetics preferredAbility to evaluate the technical results of experiments and to identify problems when they occurExcellent communication skills (written and oral)Ability to learn new techniques from written protocolsOrganizational skills and detail orientatedAbility to work independently and as a team playerBasic understanding of biochemistry, molecular biology, and some mathematical skillsPrevious experience with analysis of next generation sequencing data considered a plus Environmental Conditions / Physical Demands:Physical: Lifting up to 30 lbs occasionally, standing, bending, repetitive movement, etc.Environmental: Exposure to a wet lab environment, bloodborne pathogens, toxic/caustic chemicals or reagents. PAVIR engages in nonprofit medical research and works with sponsors and the Veterans Affairs Palo Alto Health Care System (VAPAHCS) in administering funds for conducting research to find new and improved ways to combat human disease and help people with disabilities. Please refer to our website for additional information: www.pavir.org PAVIR is pleased to be an Equal Opportunity Employer for Minorities, Females, Protected Veterans, and Qualified Individuals with a Disability. If you need special assistance or an accommodation to apply for a posted position, please contact jobs@pavir.org - Human Resources department. How to Apply: Please apply online for job code andlsquo;MAL1701.17andrsquo; via our Jobs page and include your cover